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Xiaoming HU,Jing GUO,Chunling BAI,Zhuying WEI,Li GAO,Tingmao HU,Shorgan BOU,Guangpeng LI
Frontiers of Agricultural Science and Engineering 2016, Volume 3, Issue 1, Pages 87-96 doi: 10.15302/J-FASE-2016087
Keywords: safety of transgenic bicistron gene transfer body transgenic mice muscle development
Liangyun Zhou,Guang Yang,Haifeng Sun,Jinfu Tang,Jian Yang,Yizhan Wang,Thomas Avery Garran,Lanping Guo
Frontiers of Medicine 2017, Volume 11, Issue 1, Pages 137-146 doi: 10.1007/s11684-016-0486-3
This study aims to elucidate the underlying molecular mechanisms of artemisinin accumulation induced by cadmium (Cd). The effects of different Cd concentrations (0, 20, 60, and 120 μmol/L) on the biosynthesis of Artemisia annua L. were examined. Intermediate and end products were quantified by HPLC-ESI-MS/MS analysis. The expression of key biosynthesis enzymes was also determined by qRT-PCR. The results showed that the application of treatment with 60 and 120 μmol/L Cd for 3 days significantly improved the biosynthesis of artemisinic acid, arteannuin B, and artemisinin. The concentrations of artemisinic acid, arteannuin B, and artemisinin in the 120 μmol/L Cd-treated group were 2.26, 102.08, and 33.63 times higher than those in the control group, respectively. The concentrations of arteannuin B and artemisinin in 60 μmol/L Cd-treated leaves were 61.10 and 26.40 times higher than those in the control group, respectively. The relative expression levels of HMGR, FPS, ADS, CYP71AV1, DBR2, ALDH1, and DXR were up-regulated in the 120 μmol/L Cd-treated group because of increased contents of artemisinic metabolites after 3 days of treatment. Hence, appropriate doses of Cd can increase the concentrations of artemisinic metabolites at a certain time point by up-regulating the relative expression levels of key enzyme genes involved in artemisinin biosynthesis.
Keywords: Cd secondary metabolites gene expressions Artemisia annua L.
Xia Jiahui
Strategic Study of CAE 2000, Volume 2, Issue 11, Pages 1-11
In this paper, the study on family collection, gene mapping, gene identification and gene functiont (1; 3) (q44; p11) associated with nasopharyngeal cancer was found in 1975 at first, and human TDF geneIn 1998, GJB3, a human genetic neurological deafness gene, was identified using a novel strategy of “Gene Family-Candidate Disease Gene Cloning”,and the paper was published in Nature Geneticswas also cloned from gene function study.
Keywords: genetic disease gene mapping and cloning gene familiy-candidate disease gene cloning genome wide scan gene function study
Lihui WANG, Lianhong LI, Shen LV, Shujun FAN, Li ZHAN, Bo WANG, Zhong ZHANG
Frontiers of Medicine 2009, Volume 3, Issue 2, Pages 164-170 doi: 10.1007/s11684-009-0038-1
Keywords: epithelial-mesenchymal transition vascular endothelial cell growth factor matrix metalloproteinase-9 cyclooxygenase-2 higher microvascular density breast cancer
Molecular engineering of dendrimer nanovectors for siRNA delivery and gene silencing
Yu Cao, Xiaoxuan Liu, Ling Peng
Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 4, Pages 663-675 doi: 10.1007/s11705-017-1623-5
Keywords: gene therapy RNAi therapeutics dendrimer nanovectors gene silencing
The antibiotic resistome: gene flow in environments, animals and human beings
Yongfei Hu, George F. Gao, Baoli Zhu
Frontiers of Medicine 2017, Volume 11, Issue 2, Pages 161-168 doi: 10.1007/s11684-017-0531-x
Keywords: antibiotic resistance resistome microbiome gene flow
Reflections on the system of evaluation of gene-edited livestock
Ziyao FAN, Tianwen WU, Kui WU, Yulian MU, Kui LI
Frontiers of Agricultural Science and Engineering 2020, Volume 7, Issue 2, Pages 211-217 doi: 10.15302/J-FASE-2019303
Keywords: evaluation gene editing livestock performance safety
Frontiers of Medicine 2022, Volume 16, Issue 4, Pages 627-636 doi: 10.1007/s11684-020-0815-4
Keywords: RUNX1 gene mutation acute myeloid leukemia transcriptional repression DNA methylation
Construction of lentiviral vector carrying Rab9 gene and its expression in mouse brain
Youguo HAO, Min ZHANG, Jinzhi XU, Bitao BU, Jiajun WEI
Frontiers of Medicine 2009, Volume 3, Issue 2, Pages 141-147 doi: 10.1007/s11684-009-0041-6
Keywords: Rab9 lentivirus gene therapy gene transfer
Nan Zheng, Ziyuan Song, Yang Liu, Lichen Yin, Jianjun Cheng
Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 4, Pages 521-528 doi: 10.1007/s11705-017-1612-8
Keywords: α-helical polypeptide plant gene delivery protoplast intact leaves transfection
Profiling influences of gene overexpression on heterologous resveratrol production in
Duo Liu,Bingzhi Li,Hong Liu,Xuejiao Guo,Yingjin Yuan
Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 1, Pages 117-125 doi: 10.1007/s11705-016-1601-3
Keywords: resveratrol aromatic amino acid DNA assembly metabolic engineering gene overexpression
Stem cell gene therapy: the risks of insertional mutagenesis and approaches to minimize genotoxicity
Chuanfeng Wu, Cynthia E. Dunbar
Frontiers of Medicine 2011, Volume 5, Issue 4, Pages 356-371 doi: 10.1007/s11684-011-0159-1
Keywords: gene therapy hematopoietic stem cells insertional mutagenesis genotoxicity induced pluripotent stem cell
Optimized human factor IX expression cassettes for hepatic-directed gene therapy of hemophilia B
Ru Zhang,Qiang Wang,Lin Zhang,Saijuan Chen
Frontiers of Medicine 2015, Volume 9, Issue 1, Pages 90-99 doi: 10.1007/s11684-015-0390-2
Gene therapy provides a potential cure for hemophilia B, and significant progress has been achievedin liver-directed gene transfer mediated by adeno-associated viral vectors.vectors and hFIX expression cassette may positively contribute to the ultimate success of hemophilia B geneIn addition, the regulatory elements that control the FIX gene expression in these cassettesreconstructs of hFIX with AAV vector may potentially contribute to the creation of an efficacious gene
Keywords: factor IX hemophilia B liver-specific regulatory elements hydrodynamic gene transfer
Lu Wang
Frontiers of Medicine 2013, Volume 7, Issue 3, Pages 280-289 doi: 10.1007/s11684-013-0265-3
Many gene fusions have been recognized as important diagnostic and/or prognostic markers in humanIn recent years, novel gene fusions have been identified in cases without prior knowledge of the geneticThis review focused on advanced genome-wide screening approaches in fusion gene identification, suchas microarray-based approaches, next-generation sequencing, and NanoString nCounter gene expression systemThe fundamental rationale and strategy for fusion gene identification using each biotech platform are
Keywords: gene fusion cancer microarray next-generation sequencing NanoString nCounter system
Potential functions of esophageal cancer-related gene-4 in the cardiovascular system
Rui Zhou, Yuanshu Liu, Wenjun Huang, Xitong Dang
Frontiers of Medicine 2019, Volume 13, Issue 6, Pages 639-645 doi: 10.1007/s11684-019-0701-0
Keywords: tumor suppressor gene esophageal cancer-related gene-4 cardiovascular disease hypoxia
Title Author Date Type Operation
Matrix attachment regions included in a bicistronic vector enhances and stabilizes follistatin gene expressions
Xiaoming HU,Jing GUO,Chunling BAI,Zhuying WEI,Li GAO,Tingmao HU,Shorgan BOU,Guangpeng LI
Journal Article
Effects of different doses of cadmium on secondary metabolites and gene expression in Artemisia annua
Liangyun Zhou,Guang Yang,Haifeng Sun,Jinfu Tang,Jian Yang,Yizhan Wang,Thomas Avery Garran,Lanping Guo
Journal Article
Study on Family Collection, Gene Mapping, Gene Identification and Gene Function of Human Genetic Diseases
Xia Jiahui
Journal Article
Lymphatic metastasis is related to the epithelial-mesenchymal transition and expressions of VEGF, MMP
Lihui WANG, Lianhong LI, Shen LV, Shujun FAN, Li ZHAN, Bo WANG, Zhong ZHANG
Journal Article
Molecular engineering of dendrimer nanovectors for siRNA delivery and gene silencing
Yu Cao, Xiaoxuan Liu, Ling Peng
Journal Article
The antibiotic resistome: gene flow in environments, animals and human beings
Yongfei Hu, George F. Gao, Baoli Zhu
Journal Article
Reflections on the system of evaluation of gene-edited livestock
Ziyao FAN, Tianwen WU, Kui WU, Yulian MU, Kui LI
Journal Article
Distinct gene expression pattern of mutations coordinated by target repression and promoter hypermethylation
Journal Article
Construction of lentiviral vector carrying Rab9 gene and its expression in mouse brain
Youguo HAO, Min ZHANG, Jinzhi XU, Bitao BU, Jiajun WEI
Journal Article
Gene delivery into isolated
Nan Zheng, Ziyuan Song, Yang Liu, Lichen Yin, Jianjun Cheng
Journal Article
Profiling influences of gene overexpression on heterologous resveratrol production in
Duo Liu,Bingzhi Li,Hong Liu,Xuejiao Guo,Yingjin Yuan
Journal Article
Stem cell gene therapy: the risks of insertional mutagenesis and approaches to minimize genotoxicity
Chuanfeng Wu, Cynthia E. Dunbar
Journal Article
Optimized human factor IX expression cassettes for hepatic-directed gene therapy of hemophilia B
Ru Zhang,Qiang Wang,Lin Zhang,Saijuan Chen
Journal Article
Identification of cancer gene fusions based on advanced analysis of the human genome or transcriptome
Lu Wang
Journal Article